Smad1 [p Ser206] Antibody

Validated:

Unconjugated

Polyclonal Rabbit IgG

Please see the vial label for concentration. If unlisted please contact technical services.

This affinity purified Smad1 [p Ser206] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 206 of human Smad1 protein. (Uniprot: Q15797)

A BLAST analysis was used to suggest 100% cross reactivity with Smad1 from human, Duckbill platypus, Drosophila, mouse, opossum, chimpanzee, bonobo, orangutan, gorilla, macaque, cattle, sheep, pig, rat, horse, salmon, chicken, and dog based on the sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

p Ser206

This affinity-purified antibody is directed against the phosphorylated form of human Smad1 protein at the pS206 residue. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad1 pS206 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad1 is minimal by ELISA and western blot. A BLAST analysis was used to suggest 100% cross reactivity with Smad1 from human, Duckbill platypus, Drosophila, mouse, opossum, chimpanzee, bonobo, orangutan, gorilla, macaque, cattle, sheep, pig, rat, horse, salmon, chicken, and dog based on the sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Polyclonal

Rabbit

IgG

This affinity-purified antibody is directed against the phosphorylated form of human SMAD1 protein at the [p Ser206] residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Immunogen affinity purified

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01% Sodium Azide

Please see the vial label for concentration. If unlisted please contact technical services.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.