Smad3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20411

Novus Biologicals, part of Bio-Techne
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NBP2-20411
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (99%), Canine (100%), Chicken (99%), Porcine (100%), Rhesus Macaque (100%), Xenopus (95%). Backed by our 100% Guarantee.

Applications

Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Smad3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMAD3. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (95%).

Localization

Cytoplasm (By similarity); Nucleus (By similarity)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Smad3 Antibody

Western Blot: Smad3 Antibody [NBP2-20411]

Western Blot: Smad3 Antibody [NBP2-20411]

Western Blot: Smad3 Antibody [NBP2-20411] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Smad3 Antibody [NBP2-20411]

Immunocytochemistry/ Immunofluorescence: Smad3 Antibody [NBP2-20411]

Immunocytochemistry/Immunofluorescence: SMAD3 Antibody [NBP2-20411] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Smad3 Antibody [NBP2-20411]

Immunohistochemistry-Paraffin: Smad3 Antibody [NBP2-20411]

Immunohistochemistry-Paraffin: Smad3 Antibody [NBP2-20411] - Human gastric cancer. SMAD2 / SMAD3 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
View 4 more images

Applications for Smad3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:1000-1:1000

Western Blot

1:1000-1:20000
Application Notes
ChIP, Flow- assay Assay-dependent dilution

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Smad3

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq]

Long Name

Mothers Against DPP Homolog 3

Alternate Names

DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), Mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3

Gene Symbol

SMAD3

UniProt

P84022

Additional Smad3 Products

Product Documents for Smad3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Smad3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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