Smad4 Antibody (PD01-37)

Western Blot: Smad4 Antibody (PD01-37) [NBP3-32956]

Western Blot: Smad4 Antibody (PD01-37) [NBP3-32956] - Western blot analysis of Smad4 on different lysates with Rabbit anti-Smad4 antibody (NBP3-32956) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: Ramos cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: C6 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 60 kDa
Observed band size: 60 kDa

Exposure time: 25 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32956) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Smad4 Antibody (PD01-37) [NBP3-32956]

Immunohistochemistry: Smad4 Antibody (PD01-37) [NBP3-32956] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-Smad4 antibody (NBP3-32956) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32956) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Smad4 Antibody (PD01-37) [NBP3-32956]

Immunocytochemistry/ Immunofluorescence: Smad4 Antibody (PD01-37) [NBP3-32956] - Immunocytochemistry analysis of PANC-1 cells labeling Smad4 with Rabbit anti-Smad4 antibody (NBP3-32956) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Smad4 antibody (NBP3-32956) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.