Smad4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24951
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Monkey
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was developed against synthetic peptides corresponding to amino acids 186-223 and amino acids 509-544 of human SMAD4 (GenBank Accession No. NP_005350.1). These peptide sequences are specific to SMAD4, and have high homology to SMAD4 in multiple species.
Reactivity Notes
Bovine reactivity reported from a verified customer review.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Smad4 Antibody
![Western Blot: Smad4 Antibody [NBP2-24951]](https://resources.bio-techne.com/images/products/SMAD4-Antibody-Western-Blot-NBP2-24951-img0001.jpg "Western Blot: Smad4 Antibody [NBP2-24951]")
Western Blot: Smad4 Antibody [NBP2-24951]
Western Blot: Smad4 Antibody [NBP2-24951] - SMAD4 Antibody [NBP2-24951] - Analysis using SMAD4 antibody. Lysate from A549 cells probed with SMAD4 antibody at 2 ug/mL.![Immunocytochemistry/ Immunofluorescence: Smad4 Antibody [NBP2-24951]](https://resources.bio-techne.com/images/products/Smad4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24951-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Smad4 Antibody [NBP2-24951]")
Immunocytochemistry/ Immunofluorescence: Smad4 Antibody [NBP2-24951]
Immunocytochemistry/Immunofluorescence: Smad4 Antibody [NBP2-24951] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-SMAD4 Antibody NBP2-24951 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.![Immunohistochemistry-Paraffin: Smad4 Antibody [NBP2-24951]](https://resources.bio-techne.com/images/products/Smad4-Antibody-Immunohistochemistry-Paraffin-NBP2-24951-img0002.jpg "Immunohistochemistry-Paraffin: Smad4 Antibody [NBP2-24951]")
Immunohistochemistry-Paraffin: Smad4 Antibody [NBP2-24951]
Immunohistochemistry-Paraffin: Smad4 Antibody [NBP2-24951] - Analysis of a FFPE tissue section of human colon using 1:200 dilution of SMAD4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Apical cytoplasmic staining was observed in both glandular and epithelial cells.Applications for Smad4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1 - 5 ug/mL
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
1 - 3 ug/mL
Reviewed Applications
Read 3 reviews rated 3.7 using NBP2-24951 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Smad4
Long Name
Mothers Against DPP Homolog 4
Alternate Names
DPC4, MADH4
Gene Symbol
SMAD4
UniProt
Additional Smad4 Products
Product Documents for Smad4 Antibody
Product Specific Notices for Smad4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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