Smad5 [p Ser465, p Ser463] Antibody (8F4B10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33557
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 8F4B10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic phosphorylated peptide around S463 & S465 of human Smad5 (Q99717).
Sequence:
SSVS
Sequence:
SSVS
Modification
p Ser 463, p Ser 465
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Smad5 [p Ser465, p Ser463] Antibody (8F4B10)
Western Blot: Smad5 [p Ser465, p Ser463] Antibody (8F4B10) [NBP3-33557] -
Western Blot: Smad5 [p Ser465, p Ser463] Antibody (8F4B10) [NBP3-33557] - Western blot analysis of lysates from HeLa cells, using Smad5 Rabbit mAb at 1:1000 dilution or Smad5 antibody. Hela cells were treated by ATP(5 mM) at 30C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 3min.
Applications for Smad5 [p Ser465, p Ser463] Antibody (8F4B10)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Smad5
Long Name
Mothers Against DPP Homolog 5
Alternate Names
Dwfc, JV5-1, MADH5
Gene Symbol
SMAD5
Additional Smad5 Products
Product Documents for Smad5 [p Ser465, p Ser463] Antibody (8F4B10)
Product Specific Notices for Smad5 [p Ser465, p Ser463] Antibody (8F4B10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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