Smad5 [p Ser463] Antibody (SR2030)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21515
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SR2030
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human phospho-Smad5 [Ser463, Ser465] (Uniprot #: Q99717 )
Modification
p Ser 463
Specificity
Detects endogenous levels of total phospho-Smad5 [Ser463, Ser465]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Smad5 [p Ser463] Antibody (SR2030)
Western Blot: Smad5 [p Ser 463] Antibody (SR2030) [NBP3-21515] -
Western Blot: Smad5 [p Ser 463] Antibody (SR2030) [NBP3-21515] - Western blot analysis using NBP3-21515 in (1) Mouse brain tissue lysate; (2) Rat brain tissue lysate.Immunohistochemistry: Smad5 [p Ser 463] Antibody (SR2030) [NBP3-21515] -
Immunohistochemistry: Smad5 [p Ser 463] Antibody (SR2030) [NBP3-21515] - Immunohistochemical analysis of paraffin-embedded human pancreas, using NBP3-21515.Applications for Smad5 [p Ser463] Antibody (SR2030)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Smad5
Long Name
Mothers Against DPP Homolog 5
Alternate Names
Dwfc, JV5-1, MADH5
Gene Symbol
SMAD5
Additional Smad5 Products
Product Documents for Smad5 [p Ser463] Antibody (SR2030)
Product Specific Notices for Smad5 [p Ser463] Antibody (SR2030)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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