Smad7 Antibody (3G8)
Novus Biologicals, part of Bio-Techne | Catalog # H00004092-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3G8
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SMAD7 (NP_005895, 160 a.a. ~ 260 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CKVFRWPDLRHSSEVKRLCCCESYGKINPELVCCNPHHLSRLCELESPPPPYSRYPMDFLKPTADCPDAVPSSAETGGTNYLAPGGLSDSQLLLEPGDRSH
Specificity
SMAD7 - SMAD, mothers against DPP homolog 7 (Drosophila)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Smad7 Antibody (3G8)
Immunocytochemistry/ Immunofluorescence: Smad7 Antibody (3G8) [H00004092-M01]
Immunocytochemistry/Immunofluorescence: Smad7 Antibody (3G8) [H00004092-M01] - Analysis of monoclonal antibody to SMAD7 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: Smad7 Antibody (3G8) [H00004092-M01] - Detection limit for recombinant GST tagged SMAD7 is approximately 0.1ng/ml as a capture antibody.
Applications for Smad7 Antibody (3G8)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Smad7
Long Name
Mothers Against DPP Homolog 7
Alternate Names
MADH7, MADH8
Entrez Gene IDs
4092 (Human)
Gene Symbol
SMAD7
OMIM
602932 (Human)
UniProt
Additional Smad7 Products
Product Documents for Smad7 Antibody (3G8)
Product Specific Notices for Smad7 Antibody (3G8)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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