Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SMC1 Antibodies »

Product Specifications

Immunogen

Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 957 according to the numbering given in entry (NP_006297.2)

Modification

p Ser957

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

143 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMC1 [p Ser957] Antibody

Western Blot: SMC1 [p Ser957] Antibody [NB100-205]

Western Blot: SMC1 [p Ser957] Antibody [NB100-205]

Western Blot: SMC1 [p Ser957] Antibody [NB100-205] - Whole cell extracts from HeLa cells (30 ug/lane) that were irradiated with 10 Gy of ionizing radiation or mock irradiated. Irradiated lysate was then treated with calf intestinal phosphatase (CIP) or left untreated. Antibody used at 0.1 ug/ml.
SMC1 [p Ser957] Antibody [NB100-205] - Whole cell lysate (50 ug) from 293T cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit antiPhospho SMC1 (S957) antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for SMC1 [p Ser957] Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 6 ug/mg of lysate

Western Blot

1:200-1:2
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMC1

Involved in chromosome cohesion during cell cycle and in DNA repair and is a central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. SMC1 is involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, and works as a downstream effector in the ATM/NBS1 branch of S-phase checkpoint.

Long Name

Structural Maintenance of Chromosomes 1

Alternate Names

SB1.8, SMC1A, SMC1L1

Entrez Gene IDs

8243 (Human)

Gene Symbol

SMC1A

UniProt

Q14683

Additional SMC1 Products

Product Documents for SMC1 [p Ser957] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMC1 [p Ser957] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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