SMC1 [p Ser966] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-206
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 966 according to the numbering given in entry NP_006297.2.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID:25820262).
Modification
p Ser966
Localization
Nuclear
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-204
Scientific Data Images for SMC1 [p Ser966] Antibody
Western Blot: SMC1 [p Ser966] Antibody [NB100-206]
Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes. For detection of total SMC1, rabbit anti-SMC1 antibody was used.Immunocytochemistry/ Immunofluorescence: SMC1 [p Ser966] Antibody [NB100-206]
Immunocytochemistry/Immunofluorescence: SMC1 [p Ser966] Antibody [NB100-206] - Neocarzinostatin treated asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight (R) 594 conjugated used at a dilution of 1:100.Immunohistochemistry-Paraffin: SMC1 [p Ser966] Antibody [NB100-206]
Immunohistochemistry-Paraffin: SMC1 [p Ser966] Antibody [NB100-206] - Human breast carcinoma. Antibody: Affinity purified rabbit antiPhospho SMC1 (S966) used at a dilution of 1:1,000 (1ug/ml). Detection: DABApplications for SMC1 [p Ser966] Antibody
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:500 - 1:5000
Immunohistochemistry
1:1000 - 1:5000
Immunohistochemistry-Paraffin
1:1000 - 1:5000
Immunoprecipitation
2 - 5 ug/mg of lysate
Western Blot
1:2000 - 1:10000
Application Notes
Elisa reactivity reported in (PMID: 20080624). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC/IF-Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Reviewed Applications
Read 1 review rated 5 using NB100-206 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SMC1
Long Name
Structural Maintenance of Chromosomes 1
Alternate Names
SB1.8, SMC1A, SMC1L1
Entrez Gene IDs
8243 (Human)
Gene Symbol
SMC1A
UniProt
Additional SMC1 Products
Product Specific Notices for SMC1 [p Ser966] Antibody
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-204
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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