SMC3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-207
![Western Blot: SMC3 Antibody [NB100-207] Western Blot: SMC3 Antibody [NB100-207]](https://resources.bio-techne.com/images/products/SMC3-Antibody-Western-Blot-NB100-207-img0026.jpg)
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, C. elegans
Cited:
Human, Nematode - Caenorhabditis elegans
Predicted:
Bovine (100%), Primate (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Chemotaxis, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1175 and the C-terminus (residue 1217) of human Structural Maintenance of Chromosomes 3 using the numbering given in entry NP_005436.1 (GeneID 9126).
Reactivity Notes
Orangutan (100%). C. elegans reactivity reported in scientific literature (PMID: 30563860).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SMC3 Antibody - BSA Free
Western Blot: SMC3 Antibody [NB100-207]
Western Blot: SMC3 Antibody [NB100-207] - Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, LNCaP, U2OS, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinitypurified rabbit anti-SMC3 antibody used for WB at 0.04 ug/ml. Detection:Chemiluminescence with an exposure time of 3 seconds.Immunohistochemistry-Paraffin: SMC3 Antibody [NB100-207]
Immunohistochemistry-Paraffin: SMC3 Antibody [NB100-207] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SMC3 used at 1:5,000 (0.2ug/ml). Detection: DABImmunohistochemistry-Paraffin: SMC3 Antibody [NB100-207]
Immunohistochemistry-Paraffin: SMC3 Antibody [NB100-207] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SMC3 used at 1:5,000 (0.2ug/ml). Detection: DABApplications for SMC3 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
2-10 ul/mg
Western Blot
1:10000 - 1:25000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SMC3
Alternate Names
Bamacan, BAMSMC protein 3, Basement membrane-associated chondroitin proteoglycan, BMH, Chondroitin sulfate proteoglycan 6, chondroitin sulfate proteoglycan 6 (bamacan), Chromosome-associated polypeptide, CSPG6structural maintenance of chromosomes protein 3, hCAP, HCAPbamacan, SMC-3, SMC3L1CDLS3, structural maintenance of chromosomes 3
Entrez Gene IDs
9126 (Human)
Gene Symbol
SMC3
UniProt
Additional SMC3 Products
Product Documents for SMC3 Antibody - BSA Free
Product Specific Notices for SMC3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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