SMC3 [p Ser103] Antibody

Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694]

Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694] - Detection of human Phospho SMC3 (S1083) by western blot (WB). Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE and/or 100 uM phosphatase for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody NBP2-37694 used for WB at 1 ug/ml (upper panel); to detect total SMC3, another rabbit anti-SMC3 was used for WB at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 10 seconds (lower panel).

Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694]

Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694] - Whole cell lysate (50 ug) from HeLa cells treated with 500 uM EPE for 4 hours (+) or mock treated (-) cells Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody used for WB at 1 ug/ml (upper panel); to detect total SMC3, rabbit anti-SMC3 was used for WB at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 30 seconds (lower panel).

Immunoprecipitation: SMC3 [p Ser103] Antibody [NBP2-37694]

Immunoprecipitation: SMC3 [p Ser103] Antibody [NBP2-37694] - Whole cell lysate (1mg for IP; 20% of IP loaded) from HeLa cells treated with 500 uM EPE for 4 hours (+). Antibodies: Phospho SMC3 was immunoprecipitated (IP) using rabbit anti-Phospho SMC3 (S1083) antibody. The IP reactions were either mock treated (-) or treated with phosphatases (+). Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody was used for western blot (WB) of IPs at 1 ug/ml (upper panel); to detect total SMC3 affinity purified rabbit anti-SMC3 was used for WB of IPs at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) and 30 seconds (lower panel).