SMC4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-375
![Western Blot: SMC4 Antibody [NB100-375]](https://resources.bio-techne.com/images/products/SMC4-Antibody-Western-Blot-NB100-375-img0005.jpg "Western Blot: SMC4 Antibody [NB100-375]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide, which represented a portion of human structural maintenance of chromosomes 4 encoded within exon 23 (LocusLink ID 10051). According to Swiss-Prot entry Q9NTJ3, the peptide is near the C-terminus.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SMC4 Antibody
Western Blot: SMC4 Antibody [NB100-375]
Western Blot: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.Applications for SMC4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200 - 1:400
Immunohistochemistry
1:200-1:400
Immunohistochemistry-Paraffin
1:200-1:400
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:1000-1:10000
Application Notes
NB 100-375 may be used for Western Blot, where a band at ~170 kDa is seen, representing SMC4 protein.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SMC4
Alternate Names
CAP-C, CAPCSMC4 structural maintenance of chromosomes 4-like 1 (yeast), Chromosome-associated polypeptide C, hCAP-CSMC4 (structural maintenance of chromosomes 4, yeast)-like 1, SMC protein 4, SMC-4, SMC4 structural maintenance of chromosomes 4-like 1, SMC4L1, structural maintenance of chromosomes 4, structural maintenance of chromosomes protein 4, XCAP-C homolog
Gene Symbol
SMC4
UniProt
Additional SMC4 Products
Product Documents for SMC4 Antibody
Product Specific Notices for SMC4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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