Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all SMC5 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1050 and the C-terminus (residue 1101) of human Structural Maintenance of Chromosomes 5 using the numbering given in TrEMBL entry Q8IY18 (GeneID 23137).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMC5 Antibody - BSA Free

Western Blot: SMC5 Antibody [NB100-469]

Western Blot: SMC5 Antibody [NB100-469]

Western Blot: SMC5 Antibody [NB100-469] - Detection of human and mouse SMC5 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, RKO, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC5 antibody NB100-469 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: SMC5 Antibody [NB100-469]

Immunoprecipitation: SMC5 Antibody [NB100-469]

Immunoprecipitation: SMC5 Antibody [NB100-469] - Detection of human SMC5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC5 antibody NB100-469 (lot 3) used for IP at 6 ug per reaction. SMC5 was also immunoprecipitated by a previous lot of this antibody (lot 2). For blotting immunoprecipitated SMC5, NB100-469 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for SMC5 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 28302748).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMC5

SMC (Structural maintenance of chromosomes) proteins are members of multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. Two of these complexes, cohesin and condensin consist of a core complex of an SMC1 and SMC3 heterodimer or an SMC2 and SMC4 heterodimer, respectively. A third complex includes a heterodimer of SMC5 and SMC6 whose function is not fully understood but is proposed to be related to DNA repair.

Alternate Names

hSMC5, KIAA0594SMC5 structural maintenance of chromosomes 5-like 1 (yeast), SMC protein 5, SMC-5, SMC5L1SMC5 structural maintenance of chromosomes 5-like 1, structural maintenance of chromosomes 5, structural maintenance of chromosomes protein 5

Entrez Gene IDs

23137 (Human)

Gene Symbol

SMC5

UniProt

Q8IY18 (Human)

Additional SMC5 Products

Product Documents for SMC5 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMC5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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