Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SMC5 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1050 and the C-terminus (residue 1101) of human Structural Maintenance of Chromosomes 5 using the numbering given in TrEMBL entry Q8IY18 (GeneID 23137).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMC5 Antibody

Western Blot: SMC5 Antibody [NB100-469]

Western Blot: SMC5 Antibody [NB100-469]

Western Blot: SMC5 Antibody [NB100-469] - Detection of human and mouse SMC5 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, RKO, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC5 antibody NB100-469 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: SMC5 Antibody [NB100-469]

Immunoprecipitation: SMC5 Antibody [NB100-469]

Immunoprecipitation: SMC5 Antibody [NB100-469] - Detection of human SMC5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC5 antibody NB100-469 (lot 3) used for IP at 6 ug per reaction. SMC5 was also immunoprecipitated by a previous lot of this antibody (lot 2). For blotting immunoprecipitated SMC5, NB100-469 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for SMC5 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 28302748).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMC5

SMC (Structural maintenance of chromosomes) proteins are members of multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. Two of these complexes, cohesin and condensin consist of a core complex of an SMC1 and SMC3 heterodimer or an SMC2 and SMC4 heterodimer, respectively. A third complex includes a heterodimer of SMC5 and SMC6 whose function is not fully understood but is proposed to be related to DNA repair.

Alternate Names

hSMC5, KIAA0594SMC5 structural maintenance of chromosomes 5-like 1 (yeast), SMC protein 5, SMC-5, SMC5L1SMC5 structural maintenance of chromosomes 5-like 1, structural maintenance of chromosomes 5, structural maintenance of chromosomes protein 5

Entrez Gene IDs

23137 (Human)

Gene Symbol

SMC5

UniProt

Q8IY18 (Human)

Additional SMC5 Products

Product Documents for SMC5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMC5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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