SMCHD1 Antibody (CL4270)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59046
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (97%), Rat (98%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL4270
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:DNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKED
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for SMCHD1 Antibody (CL4270)
Immunocytochemistry/ Immunofluorescence: SMCHD1 Antibody (CL4270) [NBP2-59046]
Immunocytochemistry/Immunofluorescence: SMCHD1 Antibody (CL4270) [NBP2-59046] - Staining of U-2 OS cells using the Anti-SMCHD1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4270) [NBP2-59046]
Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4270) [NBP2-59046] - Staining of human skeletal muscle shows no positivity as expected (negative control).Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4270) [NBP2-59046]
Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4270) [NBP2-59046] - Staining of human testis shows moderate to strong nuclear positivity in seminiferous tubules cells.Applications for SMCHD1 Antibody (CL4270)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SMCHD1
Alternate Names
DKFZp686O0631, KIAA0650, structural maintenance of chromosomes flexible hinge domain containing 1, structural maintenance of chromosomes flexible hinge domain-containing protein 1
Gene Symbol
SMCHD1
Additional SMCHD1 Products
Product Documents for SMCHD1 Antibody (CL4270)
Product Specific Notices for SMCHD1 Antibody (CL4270)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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