SMCHD1 Antibody (JE34-29)

Western Blot: SMCHD1 Antibody (JE34-29) [NBP3-32960]

Western Blot: SMCHD1 Antibody (JE34-29) [NBP3-32960] - Western blot analysis of SMCHD1 on different lysates with Rabbit anti-SMCHD1 antibody (NBP3-32960) at 1/1,000 dilution.

Lane 1: 293T cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HeLa cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: U-2 OS cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 226 kDa
Observed band size: 226 kDa

Exposure time: 30 seconds; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32960) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: SMCHD1 Antibody (JE34-29) [NBP3-32960]

Immunohistochemistry: SMCHD1 Antibody (JE34-29) [NBP3-32960] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-SMCHD1 antibody (NBP3-32960) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32960) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.