SMCHD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82978

Novus Biologicals, part of Bio-Techne
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NBP1-82978
NBP1-82978-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Mouse (97%), Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SMCHD1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKED

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23143600)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMCHD1 Antibody

Western Blot: SMCHD1 Antibody [NBP1-82978]

Western Blot: SMCHD1 Antibody [NBP1-82978]

Western Blot: SMCHD1 Antibody [NBP1-82978] - Analysis in human cell lines U2OS and MCF-7 using Anti-SMCHD1 antibody. Corresponding SMCHD1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.
Immunocytochemistry/ Immunofluorescence: SMCHD1 Antibody [NBP1-82978]

Immunocytochemistry/ Immunofluorescence: SMCHD1 Antibody [NBP1-82978]

Immunocytochemistry/Immunofluorescence: SMCHD1 Antibody [NBP1-82978] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SMCHD1 Antibody [NBP1-82978]

Immunohistochemistry-Paraffin: SMCHD1 Antibody [NBP1-82978]

Immunohistochemistry-Paraffin: SMCHD1 Antibody [NBP1-82978] - Staining of human skeletal muscle shows very weak nuclear positivity in myocytes.
View 4 more images

Applications for SMCHD1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Simple Western

1:40

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMCHD1

Alternate Names

DKFZp686O0631, KIAA0650, structural maintenance of chromosomes flexible hinge domain containing 1, structural maintenance of chromosomes flexible hinge domain-containing protein 1

Gene Symbol

SMCHD1

Additional SMCHD1 Products

Product Documents for SMCHD1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SMCHD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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