SMCX Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18659
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Polyclonal antibody raised in rabbit against mouse SMCX (jumonji, AT rich interactive domain 1C), using 3 KLH-conjugated synthetic peptides containing sequences from the inner part of the protein.
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for SMCX Antibody
Western Blot: SMCX Antibody [NBP3-18659]
Western Blot: SMCX Antibody [NBP3-18659] - Figure 2. Western blot analysis using the antibody directed against SMCX Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against SMCX and with the preimmune serum (lane 1) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 175 kDa); the marker (in kDa) is shown on the left. Figure 2 shows that the additional bands at approximately 55 and 80 kDa are also present in the preimmune serum.ELISA: SMCX Antibody [NBP3-18659]
ELISA: SMCX Antibody [NBP3-18659] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against SMCX . The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:7,000.Applications for SMCX Antibody
Application
Recommended Usage
ELISA
1:500
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Whole Antiserum
Formulation
Whole antiserum
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: SMCX
Long Name
Lysine-specific demethylase 5C
Alternate Names
DXS1272E, JARID1C, KDM5C, Protein SmcX, Protein Xe169, XE169
Gene Symbol
KDM5C
UniProt
Additional SMCX Products
Product Specific Notices for SMCX Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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