SMEK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83882

Novus Biologicals, part of Bio-Techne
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NBP1-83882
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SMEK2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GQTFKGLKTKYEQEKDRQNQKLNSVPSILRSNRFRRDAKALEEDEEMWFNEDEEEEGKAVMPPLE

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMEK2 Antibody

Western Blot: SMEK2 Antibody [NBP1-83882]

Western Blot: SMEK2 Antibody [NBP1-83882]

Western Blot: SMEK2 Antibody [NBP1-83882] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: SMEK2 Antibody [NBP1-83882]

Immunocytochemistry/ Immunofluorescence: SMEK2 Antibody [NBP1-83882]

Immunocytochemistry/Immunofluorescence: SMEK2 Antibody [NBP1-83882] - Staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SMEK2 Antibody [NBP1-83882]

Immunohistochemistry-Paraffin: SMEK2 Antibody [NBP1-83882]

Immunohistochemistry-Paraffin: SMEK2 Antibody [NBP1-83882] - Analysis in human fallopian tube and skeletal muscle tissues using NBP1-83882 antibody. Corresponding PPP4R38 RNA-seq data are presented for the same tissues.
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Applications for SMEK2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMEK2

Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C atcentrosomal microtubule organizing centers

Alternate Names

FLFL2, FLJ31474, KIAA1387serine/threonine-protein phosphatase 4 regulatory subunit 3B, PP4R3B, PPP4R3B, PSY2, SMEK homolog 2, SMEK homolog 2, suppressor of mek1 (Dictyostelium), smk1

Gene Symbol

SMEK2

Additional SMEK2 Products

Product Documents for SMEK2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SMEK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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