Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SMG1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 2925 and 2975 of human PI-3-Kinase-related kinase SMG1 using the numbering given in entry NP_055907.3 (GeneID 23049).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMG1 Antibody

Western Blot: SMG1 Antibody [NB100-2321]

Western Blot: SMG1 Antibody [NB100-2321]

Western Blot: SMG1 Antibody [NB100-2321] - Detection of human SMG1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMG1 antibody NB100-2321 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Immunoprecipitation: SMG1 Antibody [NB100-2321]

Immunoprecipitation: SMG1 Antibody [NB100-2321]

Immunoprecipitation: SMG1 Antibody [NB100-2321] - Detection of human SMG1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMG1 antibody NB100-2321 (lot 2) used for IP at 6 ug per reaction. SMG1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated SMG1, NB100-2321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for SMG1 Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMG1

This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. This protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein.

Alternate Names

ATXserine/threonine-protein kinase SMG1, EC 2.7.11, EC 2.7.11.1, hSMG-1,61E3.4, KIAA0421phosphatidylinositol 3-kinase-related protein kinase, Lambda/iota protein kinase C-interacting protein, Lambda-interacting protein, LIPPI-3-kinase-related kinase SMG-1, phosphatidylinositol 3-kinase-related kinase, SMG-1, SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)

Entrez Gene IDs

23049 (Human)

Gene Symbol

SMG1

UniProt

NC_000016.8 (Human)

Additional SMG1 Products

Product Documents for SMG1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMG1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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