SMN Antibody (2B1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80955

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Primate, Xenopus, Zebrafish

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2B1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all SMN Antibodies »

Product Specifications

Immunogen

Purified recombinant His6-tagged human SMN protein. [Swiss-Prot# Q16637]

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 27273160).

Localization

Cytoplasm. Nucleus > Gem, localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies (Gems).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SMN Antibody (2B1) - Azide and BSA Free

Western Blot: SMN Antibody (2B1)Azide and BSA Free [NBP2-80955]

Western Blot: SMN Antibody (2B1)Azide and BSA Free [NBP2-80955]

Western Blot: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955] - Analysis of SMN expression in 1) HeLa, 2) NTera2, 3) HepG2, 4) MCF7, 5) NIH 3T3, 6) PC12, and 7) COS7 whole cell lysates. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955]

Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955]

Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-SMN Antibody (2B1) NBP2-80955 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955]

Immunohistochemistry: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955]

Immunohistochemistry: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955] - CBs lose their colocalization with SMN nuclear foci in diabetic mice. Normal and pathological distribution of SMN proteins in sensory neurons. SMN proteins were found throughout the cytoplasm and as nuclear foci in control sensory neurons. SMN nuclear foc
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Applications for SMN Antibody (2B1) - Azide and BSA Free

Application
Recommended Usage

ELISA

reported in scientific literature (PMID 23973875)

Flow Cytometry

reported in scientific literature (PMID 30102724)

Immunocytochemistry/ Immunofluorescence

1:250

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10-1:500

Western Blot

2 ug/ml
Application Notes
This SMN Antibody (2B1) is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry, Immunoprecipitation, and Western blot where a band can be seen at ~ 35 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMN

Spinal muscular atrophy (SMA) is a common fatal autosomal recessive disorder characterized by the degeneration of motor neurons and muscular atrophy. A gene called Survival of Motor Neurons (SMN) has been identified as the determining gene in SMA and is deleted or mutated in greater than 98% of SMA patients. The SMN protein localizes to both the cytoplasm and the nucleus. In the nucleus, SMN has been found to localize in a nuclear structure termed gems for Gemini of coiled bodies, which contain high levels of small ribonucleoproteins (snRNPs). SMN forms complexes with proteins including SIP1, Gemin 2-7 and several others known to be involved in the biogenesis of snRNPs. SMN antibodies may serve as a useful research tool to study SMN complexes and gems, and their role in pre-RNA processing and RNA metabolism.

Alternate Names

Gemin-1, Kugelberg-Welander disease), SMNC, survival motor neuron protein, survival of motor neuron 1, telomeric

Gene Symbol

SMN1

Additional SMN Products

Product Documents for SMN Antibody (2B1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SMN Antibody (2B1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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