SMN2 Antibody (1A3-2B9)

Novus Biologicals, part of Bio-Techne | Catalog # H00006607-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A3-2B9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SMN2 Antibodies »

Product Specifications

Immunogen

SMN2 (AAH00908, 1 a.a. ~ 282 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAMSSGGSGGGVPEQEDSVLFRRGTGQSDDSDIWDDTALIKAYDKAVASFKHALKNGDICETSGKPKTTPKRKPAKKNKSQKKNTAASLQQWKVGDKCSAIWSEDGCIYPATIASIDFKRETCVVVYTGYGNREEQNLSDLLSPICEVANNIEQNAQENENESQVSTDESENSRSPGNKSDNIKPKSAPWNSFLPPPPPMPGPRLGPGKPGLKFNGPPPPPPPPPPHLLSCWLPPFPSGPPIIPPPPPICPDSLDDADALGSMLISWYMSGYHTGYYMEMLA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SMN2 Antibody (1A3-2B9)

Western Blot: SMN2 Antibody (1A3-2B9) [H00006607-M02]

Western Blot: SMN2 Antibody (1A3-2B9) [H00006607-M02]

Western Blot: SMN2 Antibody (1A3-2B9) [H00006607-M02] - Analysis of SMN2 expression in transfected 293T cell line by SMN2 monoclonal antibody (M02), clone 1A3-2B9.Lane 1: SMN2 transfected lysate (Predicted MW: 31.8 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: SMN2 Antibody (1A3-2B9) [H00006607-M02]

Immunocytochemistry/ Immunofluorescence: SMN2 Antibody (1A3-2B9) [H00006607-M02]

Immunocytochemistry/Immunofluorescence: SMN2 Antibody (1A3-2B9) [H00006607-M02] - Analysis of monoclonal antibody to SMN2 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: SMN2 Antibody (1A3-2B9) [H00006607-M02] - Detection limit for recombinant GST tagged SMN2 is approximately 1ng/ml as a capture antibody.

Applications for SMN2 Antibody (1A3-2B9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SMN2

Gemin 1 (SMN) is the central component of a large oligomeric complex (SMN complex), including Gemins 2-7, that is necessary and sufficient for the in vivo assembly of Sm proteins onto the small nuclear (sn)RNAs that mediate pre-mRNA splicing. The SMN complex is found in the cytoplasm and nucleus. Within the nucleus, this protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). Spinal muscular atrophy, a motor neuron disorder, results from reduced levels or a mutation in the Gemin 1 protein.

Alternate Names

BCD541, C-BCD541, Component of gems 1, FLJ76644, gemin 1, gemin-1, MGC20996, MGC5208, SMN, SMNCSMN1, SMNT, survival motor neuron protein, survival of motor neuron 2, centromeric

Gene Symbol

SMN2

UniProt

AAH00908 (Human)

Additional SMN2 Products

Product Documents for SMN2 Antibody (1A3-2B9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SMN2 Antibody (1A3-2B9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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