Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Orangutan (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SMNDC1 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 188 and 238 of human Splicing Factor 30 using the numbering given in entry NP_005862.1 (GeneID 10285).

Reactivity Notes

X. tropicalis (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMNDC1 Antibody

Western Blot: SMNDC1 Antibody [NBP1-47302]

Western Blot: SMNDC1 Antibody [NBP1-47302]

Western Blot: SMNDC1 Antibody [NBP1-47302] - Whole cell lysates from ovarian surface epithial cells (OSE), ovarian cancer cell line A2780, breast cancer cell line MCF7. Image submitted by a verified customer review.
Western Blot: SMNDC1 Antibody [NBP1-47302]

Western Blot: SMNDC1 Antibody [NBP1-47302]

Western Blot: SMNDC1 Antibody [NBP1-47302] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SMNDC1 antibody NBP1-47302 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SPF30 was also immunoprecipitated by rabbit anti-SMNDC1 antibody NBP1-47301, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Applications for SMNDC1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
SMNDC1 antibody validated for WB from a verified customer review. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Reviewed Applications

Read 1 review rated 5 using NBP1-47302 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMNDC1

This gene is a paralog of SMN1 gene, which encodes the survival motor neuron protein, mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. The protein encoded by this gene is a nuclear protein that has been identified as a constituent of the spliceosome complex. This gene is differentially expressed, with abundant levels in skeletal muscle, and may share similar cellular function as the SMN1 gene. [provided by RefSeq]

Alternate Names

SMNRSMN-related protein, SPF30splicing factor 30, survival of motor neuron-related, survival motor neuron domain containing 1,30 kDa splicing factor SMNrp, Survival motor neuron domain-containing protein 1, survival of motor neuron-related-splicing factor 30

Entrez Gene IDs

10285 (Human)

Gene Symbol

SMNDC1

Additional SMNDC1 Products

Product Documents for SMNDC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMNDC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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