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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Orangutan (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 188 and 238 of human Splicing Factor 30 using the numbering given in entry NP_005862.1 (GeneID 10285).

Reactivity Notes

X. tropicalis (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMNDC1 Antibody - BSA Free

Western Blot: SMNDC1 Antibody [NBP1-47302]

Western Blot: SMNDC1 Antibody [NBP1-47302]

Western Blot: SMNDC1 Antibody [NBP1-47302] - Whole cell lysates from ovarian surface epithial cells (OSE), ovarian cancer cell line A2780, breast cancer cell line MCF7. Image submitted by a verified customer review.
Western Blot: SMNDC1 Antibody [NBP1-47302]

Western Blot: SMNDC1 Antibody [NBP1-47302]

Western Blot: SMNDC1 Antibody [NBP1-47302] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SMNDC1 antibody NBP1-47302 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SPF30 was also immunoprecipitated by rabbit anti-SMNDC1 antibody NBP1-47301, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Applications for SMNDC1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
SMNDC1 antibody validated for WB from a verified customer review. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Reviewed Applications

Read 1 review rated 5 using NBP1-47302 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMNDC1

This gene is a paralog of SMN1 gene, which encodes the survival motor neuron protein, mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. The protein encoded by this gene is a nuclear protein that has been identified as a constituent of the spliceosome complex. This gene is differentially expressed, with abundant levels in skeletal muscle, and may share similar cellular function as the SMN1 gene. [provided by RefSeq]

Alternate Names

SMNRSMN-related protein, SPF30splicing factor 30, survival of motor neuron-related, survival motor neuron domain containing 1,30 kDa splicing factor SMNrp, Survival motor neuron domain-containing protein 1, survival of motor neuron-related-splicing factor 30

Entrez Gene IDs

10285 (Human)

Gene Symbol

SMNDC1

Additional SMNDC1 Products

Product Documents for SMNDC1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMNDC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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