SMOC-1 Antibody (09) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26898

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 09

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SMOC-1 Antibodies »

Product Summary for SMOC-1 Antibody (09) - Azide and BSA Free

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SMOC-1 (RefSeq#: NP_001030024.1; Met1-Val435).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Applications for SMOC-1 Antibody (09) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMOC-1

SMOC-1 (secreted, or SPARC-related, modular calcium-binding protein 1) is a 70-90 kDa secreted glycoprotein that is a member of the SPARC family of matricellular molecules. Mature mouse SMOC-1 is 427 amino acids (aa) in length. It contains one Kazal-like domain (aa 42-86), two thyroglobulin type-1 segments (aa 91-157 and 223-291) and two functional EF-hand sequences (aa 358-393 and 395-430). Two splice variants contain an insertion of eleven aa after Lys174, with one of these also including an alternate start site at Met65. Mature mouse SMOC-1 shares 99% aa identity with rat SMOC-1 and 92% aa identity with human, canine and bovine SMOC-1. The principal difference between rodents and other mammals is an additional 19 aa near the C-terminus of rodent SMOC-1. Like other matricellular proteins, SMOC-1 is primarily expressed in basement membranes, although it has also been found in other extracellular matrices and the oocyte zona pellucida. It is present early in mouse embryogenesis, and is produced by cells deriving from all three germ layers. Recombinant bacterially produced human SMOC-1 and SMOC-2 were both shown to bind the acute phase protein, C-reactive protein, and the adhesion proteins, fibulin and vitronectin. A signaling role for SMOC-1 was shown in rat mesangial cells: induction of nitric oxide in response to inflammatory cytokines downregulates SMOC-1 which, in turn, downregulates expression of TGF-beta and TGF-beta-regulated genes. This mechanism is proposed to limit the profibrotic effects of TGF-beta, for example in the glomerulus. In Xenopus, the SMOC paralog has been shown to antagonize BMP activity.

Long Name

Secreted Modular Calcium-binding Protein 1

Alternate Names

SMOC1, SPARC-related modular calcium-binding protein 1

Gene Symbol

SMOC1

Additional SMOC-1 Products

Product Documents for SMOC-1 Antibody (09) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SMOC-1 Antibody (09) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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