SMPD1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22365
![Western Blot: SMPD1 Antibody [NBP2-22365]](https://resources.bio-techne.com/images/products/SMPD1-Antibody-Western-Blot-NBP2-22365-img0002.jpg "Western Blot: SMPD1 Antibody [NBP2-22365]")
![Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365]](https://resources.bio-techne.com/images/products/SMPD1-Antibody-Immunohistochemistry-Paraffin-NBP2-22365-img0005.jpg "Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365]")
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 391-419 in the C-terminal region of human SMPD1 (UniProt Accession #P17405). Species sequence homology: bovine
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Scientific Data Images for SMPD1 Antibody
![Western Blot: SMPD1 Antibody [NBP2-22365]](https://resources.bio-techne.com/images/products/SMPD1-Antibody-Western-Blot-NBP2-22365-img0004.jpg "Western Blot: SMPD1 Antibody [NBP2-22365]")
Western Blot: SMPD1 Antibody [NBP2-22365]
Western Blot: SMPD1 Antibody [NBP2-22365] - Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: A431 whole cell lysate |Lysates/proteins at 20 ug per lane. Secondary: IgG (HRP) Goat Anti-Rabbit (1:10000) Predicted band size: 70kD Blocking/Dilution buffer: 5% NFDM/TBST.![Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365]](https://resources.bio-techne.com/images/products/SMPD1-Antibody-Immunohistochemistry-Paraffin-NBP2-22365-img0006.jpg "Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365]")
Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365]
Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365] - Immunohistochemical analysis of paraffin-embedded human breast tissue using SMPD1 antibody (1:500) after HIER using EDTA buffer pH9.0 prior to staining performed on the Leica(R) BOND RXm. An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody..![Flow Cytometry: SMPD1 Antibody [NBP2-22365]](https://resources.bio-techne.com/images/products/SMPD1-Antibody-Flow-Cytometry-NBP2-22365-img0007.jpg "Flow Cytometry: SMPD1 Antibody [NBP2-22365]")
Flow Cytometry: SMPD1 Antibody [NBP2-22365]
Flow Cytometry: SMPD1 Antibody [NBP2-22365] - Indirect Flow Cytometric analysis of K562 cells (right histogram) using SMPD1 antibody compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Applications for SMPD1 Antibody
Application
Recommended Usage
ELISA
1:1000
Flow Cytometry
1:25
Immunohistochemistry
1:500
Immunohistochemistry-Paraffin
1:500
Western Blot
1:4000
Application Notes
Immunohistochemistry (FFPE): Requires HIER using EDTA, pH 8.0 buffer pH9.0 prior to staining. Optimal dilutions to be determined by the researcher.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SMPD1
Long Name
Sphingomyelin Phosphodiesterase 1, Acid Lysosomal
Alternate Names
aSMase, NPD
Gene Symbol
SMPD1
UniProt
Additional SMPD1 Products
Product Documents for SMPD1 Antibody
Product Specific Notices for SMPD1 Antibody
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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