SMPD4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33567
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LAQLITQAKHTAKSISDQCAESPAGHSFLSWLGFSSMDTNGSYTANDLDEMGQDSVRKTDEYLEKALEYLRQIFRLSEAQLRQFTLALGTTQDENGKKQLPDCI
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (84%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SMPD4 Antibody
Immunocytochemistry/ Immunofluorescence: SMPD4 Antibody [NBP2-33567]
Immunocytochemistry/Immunofluorescence: SMPD4 Antibody [NBP2-33567] - Staining of human cell line MCF7 shows localization to nuclear membrane. Antibody staining is shown in green.Immunohistochemistry-Paraffin: SMPD4 Antibody [NBP2-33567]
Immunohistochemistry-Paraffin: SMPD4 Antibody [NBP2-33567] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.Immunohistochemistry-Paraffin: SMPD4 Antibody [NBP2-33567]
Immunohistochemistry-Paraffin: SMPD4 Antibody [NBP2-33567] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.Applications for SMPD4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SMPD4
Alternate Names
KIAA1418, NET13, Neutral Sphingomyelinase 3, Neutral Sphingomyelinase III, NSMASE3, NSMASE-3, SKNY Protein, Sphingomyelin Phosphodiesterase 4, Sphingomyelin Phosphodiesterase 4, Neutral Membrane (Neutral Sphingomyelinase-3)
Gene Symbol
SMPD4
UniProt
Additional SMPD4 Products
Product Documents for SMPD4 Antibody
Product Specific Notices for SMPD4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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