SMYD3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20432
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (93%), Porcine (95%), Rat (95%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Applications
Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for SMYD3 Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human SMYD3. The exact sequence is proprietary.
Reactivity Notes
Bovine (83%).
Localization
Cytoplasm; Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SMYD3 Antibody
![Western Blot: SMYD3 Antibody [NBP2-20432]](https://resources.bio-techne.com/images/products/SMYD3-Antibody-Western-Blot-NBP2-20432-img0004.jpg "Western Blot: SMYD3 Antibody [NBP2-20432]")
Western Blot: SMYD3 Antibody [NBP2-20432]
Western Blot: SMYD3 Antibody [NBP2-20432] - IP Sample: 293T whole cell lysate/extract A : Control with 2.5 ug of pre-immune rabbit IgG B : Immunoprecipitation of SMYD3 by 2.5 ug of SMYD3 antibody 10% SDS-PAGE The immunoprecipitated SMYD3 protein was detected by SMYD3 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.![Immunohistochemistry-Paraffin: SMYD3 Antibody [NBP2-20432]](https://resources.bio-techne.com/images/products/SMYD3-Antibody-Immunohistochemistry-Paraffin-NBP2-20432-img0002.jpg "Immunohistochemistry-Paraffin: SMYD3 Antibody [NBP2-20432]")
Immunohistochemistry-Paraffin: SMYD3 Antibody [NBP2-20432]
Immunohistochemistry-Paraffin: SMYD3 Antibody [NBP2-20432] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.
Chromatin Immunoprecipitation: SMYD3 Antibody [NBP2-20432] - Cross-linked ChIP was performed with HepG2 chromatin extract and 5 ug of either control rabbit IgG or anti-SMYD3 antibody. The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8.
Applications for SMYD3 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SMYD3
Long Name
Histone-lysine N-methyltransferase SMYD3
Alternate Names
ZMYND1, ZNFN3A1
Gene Symbol
SMYD3
Additional SMYD3 Products
Product Documents for SMYD3 Antibody
Product Specific Notices for SMYD3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.