SMYD4 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93044
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_443160.2). MDLPVDEWKSYLLQKWASLPTSVQVTISTAETLRDIFLHSSSLLQPEDELFLKRLSKGYLVGKDSDAPLFYREEGNKKFQEKDYTGAAVLYSKGVSHSRPNTEDMSLCHANRSAALFHLGQYETCLKDINRAQTHGYPERLQPKIMLRKAECLVALGRLQEASQTISDLERNFTATPALADVLPQTLQRNLHRLKMKMQEKDSLTESFPAALAKTLEDAALREENEQLSNASSSIGLCVDPLKGRCLVATKDILPGELLV
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SMYD4 Antibody - BSA Free
Western Blot: SMYD4 AntibodyBSA Free [NBP2-93044]
Western Blot: SMYD4 Antibody [NBP2-93044] - Analysis of extracts of various cell lines, using SMYD4 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.Immunocytochemistry/ Immunofluorescence: SMYD4 Antibody - BSA Free [NBP2-93044]
Immunocytochemistry/Immunofluorescence: SMYD4 Antibody [NBP2-93044] - Analysis of A-549 cells using SMYD4 . Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: SMYD4 Antibody - BSA Free [NBP2-93044]
Immunohistochemistry-Paraffin: SMYD4 Antibody [NBP2-93044] - Paraffin-embedded rat spleen using SMYD4 .Applications for SMYD4 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry
1:50-1:200
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:1000 - 1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SMYD4
Alternate Names
KIAA1936ZMYND21, SET and MYND domain containing 4, SET and MYND domain-containing protein 4
Gene Symbol
SMYD4
Additional SMYD4 Products
Product Documents for SMYD4 Antibody - BSA Free
Product Specific Notices for SMYD4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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