SNAP25 [p Ser187] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29522

Novus Biologicals, part of Bio-Techne
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NBP2-29522
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Guinea Pig (100%), Human (100%), Primate (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SNAP25 Antibodies »

Product Summary for SNAP25 [p Ser187] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser187 conjugated to KLH. Accession # P60881

Modification

p Ser187

Specificity

Specific for endogenous levels of the ~25 kDa SNAP25 protein phosphorylated at Ser187. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SNAP25 [p Ser187] Antibody

Western Blot: SNAP25 [p Ser187] Antibody [NBP2-29522]

Western Blot: SNAP25 [p Ser187] Antibody [NBP2-29522]

Western Blot: SNAP25 [p Ser187] Antibody [NBP2-29522] - Western blot of rat hippocampal lysate showing specific immunolabeling of the ~25k SNAP25 phosphorylated at Ser187 (Control). Phosphospecificity is shown in the right lane where the signal is completely eliminated by treatment with lambda phosphatase (lambda-Ptase, 400 units/100ul lysate for 30 min).

Applications for SNAP25 [p Ser187] Antibody

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SNAP25

SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimer's Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001). Increasing evidence suggests that SNAP-25 also modulates various ion channels, including voltage gated calcium channels (VGCCs) (Pozzi el al., 2008). Activation of PKC results in the phosphorylation of SNAP-25 on ser187 (Shu et al., 2008). Phosphorylation of SNAP25 on ser187 is believed to cause inhibition of VGCC (Pozzi el al., 2008). Since ser187 phosphorylation is transiently induced by neuronal activity, SNAP25 creates a negative feedback mechanism for controlling neuronal excitability (Pozzi el al., 2008).

Long Name

Synaptosomal-associated Protein, 25 kDa

Alternate Names

RIC4, SEC9, SUP

Entrez Gene IDs

25012 (Rat)

Gene Symbol

SNAP25

UniProt

P60881 (Rat)

Additional SNAP25 Products

Product Documents for SNAP25 [p Ser187] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SNAP25 [p Ser187] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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