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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 600 and 650 of human salt-inducible kinase 2 using the numbering given in entry NP_056006.1 (GeneID 23235).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SNF1LK2/SIK2 Antibody

Western Blot: SNF1LK2/SIK2 Antibody [NBP1-42690]

Western Blot: SNF1LK2/SIK2 Antibody [NBP1-42690]

Western Blot: SNF1LK2/SIK2 Antibody [NBP1-42690] - Detection of human SIK2 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SIK2 antibody NBP1-42690 used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: SNF1LK2/SIK2 Antibody [NBP1-42690]

Immunoprecipitation: SNF1LK2/SIK2 Antibody [NBP1-42690]

Immunoprecipitation: SNF1LK2/SIK2 Antibody [NBP1-42690] - Detection of human SIK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SIK2 antibodyNBP1-42690 used for IP at 3 ug per reaction. SIK2 was also immunoprecipitated by another rabbit anti-SIK2 antibody. For blotting immunoprecipitated SIK2, NBP1-42690 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for SNF1LK2/SIK2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SNF1LK2/SIK2

The protein Salt Inducible Kinase 2 (SIK2) has been found to play a critical role in cell division and regulating the response of ovarian cancer to chemotherapy.

Long Name

SNF1-like Kinase 2

Alternate Names

LOH11CR1I, QIK, SIK2

Entrez Gene IDs

23235 (Human)

Gene Symbol

SIK2

Additional SNF1LK2/SIK2 Products

Product Documents for SNF1LK2/SIK2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SNF1LK2/SIK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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