SNF8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32449

Novus Biologicals, part of Bio-Techne
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NBP2-32449
NBP2-32449-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SNF8 Antibodies »

Product Summary for SNF8 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: WSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLEELHQQVLKGRGKFAQDVSQDDLIRAIKKLKALGTGFGIIPVGGTYL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SNF8 Antibody

Immunocytochemistry/ Immunofluorescence: SNF8 Antibody [NBP2-32449]

Immunocytochemistry/ Immunofluorescence: SNF8 Antibody [NBP2-32449]

Immunocytochemistry/Immunofluorescence: SNF8 Antibody [NBP2-32449] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: SNF8 Antibody [NBP2-32449]

Immunohistochemistry-Paraffin: SNF8 Antibody [NBP2-32449]

Immunohistochemistry-Paraffin: SNF8 Antibody [NBP2-32449] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.

Applications for SNF8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SNF8

Component of the escrt-ii complex, which is required for multivesicular bodies (mvbs) formation and sorting of endosomal cargo proteins into mvbs. the mvb pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. the escrt-ii complex is probably involved in the recruitment of the escrt-iii complex. the escrt-ii complex may also play a role in transcription regulation by participating in derepression of transcription by rna polymerase ii, possibly via its interaction with ell.

Alternate Names

EAP30 subunit of ELL complex, EAP30hVps22, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22, SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae), vacuolar-sorting protein SNF8, VPS22Dot3

Gene Symbol

SNF8

Additional SNF8 Products

Product Documents for SNF8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SNF8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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