SNX27 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45283

Novus Biologicals, part of Bio-Techne
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NBP1-45283
NBP1-45283SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Predicted:

Bovine (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SNX27 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human SNX27 (within residues 450-500). [Swiss-Prot# Q96L92]

Reactivity Notes

Predicted to react with rat, bovine and Zebrafish based on 100% sequence homology. Use in Rat reported in scientific literature (PMID:32413996).

Localization

Cytoplasm cytosol. Early endosome. Note: In T lymphocytes, recruited from the cytosol to sorting endosomes by phosphoinositide-3-kinase products.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SNX27 Antibody - BSA Free

Western Blot: SNX27 AntibodyBSA Free [NBP1-45283]

Western Blot: SNX27 AntibodyBSA Free [NBP1-45283]

Western Blot: SNX27 Antibody [NBP1-45283] - Analysis of SNX27 on mouse brain extracts.
Immunocytochemistry/ Immunofluorescence: SNX27 Antibody - BSA Free [NBP1-45283]

Immunocytochemistry/ Immunofluorescence: SNX27 Antibody - BSA Free [NBP1-45283]

Immunocytochemistry/Immunofluorescence: SNX27 Antibody [NBP1-45283] - Detection of SNX27 (Green) in Hela cells using NBP1-45283. Nuclei (Blue) are counterstained using Hoechst 33258.
Immunohistochemistry: SNX27 Antibody - BSA Free [NBP1-45283]

Immunohistochemistry: SNX27 Antibody - BSA Free [NBP1-45283]

Immunohistochemistry: SNX27 Antibody [NBP1-45283] - IHC analysis of SNX27 in mouse bladder using DAB with hematoxylin counterstain.
View 2 more images

Applications for SNX27 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

reported in scientific literature (PMID 32413996)

Simple Western

10 ug/ml

Western Blot

1 ug/ml
Application Notes
In Western blot, a band is seen at approx. 65 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SNX27

SNX27 belongs to SNX family of proteins which are unified by the presence of a phox domain, a phospholipid-binding motif, and are involved in intracellular sorting as well as trafficking. SNX27 was initially discovered in neocortex of rat as a developmentally regulated psychostimulant-inducible novel gene, which was named mrt1 (methamphetamine-responsive transcript 1), having two splice forms mrt1a (SNX27a) and mrt1b (SNX27b) that vary only in their C-terminal ends. In SNX family, SNX27 is the only SNX member containing a PDZ domain and it interact with the PDZ-bm of a few membrane proteins through its PDZ domain to regulate their intracellular sorting. SNX27 modulates the early endosomal trafficking of 5-HT4(a)R [5-hydroxytryptamine type-4(a) receptor], Kir3 (G protein-gated potassium) channels, NR2C (N-methyl-D-aspartate receptor 2C) etc., and has also been suggested to facilitate efficient recycling of beta2AR/beta2-adrenoreceptor to the plasma membranes.

Alternate Names

KIAA0488MGC126873, methamphetamine-responsive transcript 1, MGC126871, MGC20471, MRT1, MY014, sorting nexin family member 27, sorting nexin-27

Entrez Gene IDs

81609 (Human); 76742 (Mouse)

Gene Symbol

SNX27

UniProt

Q96L92 (Human), Q3UHD6 (Mouse)

Additional SNX27 Products

Product Documents for SNX27 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SNX27 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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