SOAT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80547

Novus Biologicals, part of Bio-Techne
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NBP1-80547
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all SOAT1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide directed towards the middle region of human SOAT1 (NP_003092). Peptide sequence ASRFIIIFEQIRFVMKAHSFVRENVPRVLNSAKEKSSTVPIPTVNQYLYF. The peptide sequence for this immunogen was taken from within the described region.

Specificity

[Important Note:- ACAT1 is a symbol for both acyl-Coenzyme A: cholesterol acyltransferase 1 (SOAT1/ACAT1) and for acetyl-CoA acetyltransferase 1 (ACAT1/ THIL, Gene ID 38, Uniprot P24752) which often cause confusion].

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for SOAT1 Antibody

Western Blot: SOAT1 Antibody [NBP1-80547]

Western Blot: SOAT1 Antibody [NBP1-80547]

Western Blot: ACAT Antibody [NBP1-80547] - Hela cell lysate, concentration 0.2-1 ug/ml.

Applications for SOAT1 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOAT1

Acely-CoA:cholestorl acyltransferase (ACAT) is an integral membrane enzyme that belongs to type-B carboxylesterase/lipase family. ACAT catalyzes the formation of cholesteryl esters from cholesterol and long-chain fatty-acyl-coenzyme A (1). ACAT also function as a regulator of intracellular cholesterol homeostasis at single cell level. Two isoform of ACAT exists, ACAT1 and ACAT2, are expressed in liver and intestine. However, ACAT1 is only found in macrophages and SMCs (2). The accumulation of cholesteryl esters produced by ACAT in macrophages can lead to foam cell formation, an indication of early stage atherosclerosis. ACAT activity has been linked to Alzehimer's Diease, condition caused by excess amount of amyloid beta-peptide (Abeta). Atherogenic lipoproteins activate ACAT activity, where active ACAT can modulate the level of Abeta (3).

Long Name

Sterol O-Acyltransferase 1

Alternate Names

ACACT, ACAT-1, EC 2.3.1.26, SOAT1

Entrez Gene IDs

6646 (Human)

Gene Symbol

SOAT1

UniProt

P35610 (Human)

Additional SOAT1 Products

Product Documents for SOAT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SOAT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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