SOCS-3 Antibody (OTI3D3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00817
![Flow Cytometry: SOCS-3 Antibody (OTI3D3) [NBP2-00817]](https://resources.bio-techne.com/images/products/SOCS3-Antibody-3D3-Flow-Cytometry-NBP2-00817-img0002.jpg "Flow Cytometry: SOCS-3 Antibody (OTI3D3) [NBP2-00817]")
![Western Blot: SOCS-3 Antibody (OTI3D3) [NBP2-00817]](https://resources.bio-techne.com/images/products/SOCS3-Antibody-3D3-Western-Blot-NBP2-00817-img0003.jpg "Western Blot: SOCS-3 Antibody (OTI3D3) [NBP2-00817]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI3D3
Concentration
1 mg/ml
Product Summary for SOCS-3 Antibody (OTI3D3)
Immunogen
Full length human recombinant protein of human SOCS3 (NP_003946) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
24.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SOCS-3 Antibody (OTI3D3)
Western Blot: SOCS-3 Antibody (OTI3D3) [NBP2-00817]
Western Blot: SOCS3 Antibody (3D3) [NBP2-00817] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOCS3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOCS3.
Immunocytochemistry/Immunofluorescence: SOCS3 Antibody (3D3) [NBP2-00817] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3.
![Immunohistochemistry-Paraffin: SOCS-3 Antibody (OTI3D3) [NBP2-00817]](https://resources.bio-techne.com/images/products/SOCS3-Antibody-3D3-Immunohistochemistry-Paraffin-NBP2-00817-img0005.jpg "Immunohistochemistry-Paraffin: SOCS-3 Antibody (OTI3D3) [NBP2-00817]")
Immunohistochemistry-Paraffin: SOCS-3 Antibody (OTI3D3) [NBP2-00817]
Immunohistochemistry-Paraffin: SOCS3 Antibody (3D3) [NBP2-00817] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SOCS3 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: SOCS-3 Antibody (OTI3D3) [NBP2-00817]](https://resources.bio-techne.com/images/products/SOCS3-Antibody-3D3-Immunohistochemistry-Paraffin-NBP2-00817-img0004.jpg "Immunohistochemistry-Paraffin: SOCS-3 Antibody (OTI3D3) [NBP2-00817]")
![Flow Cytometry: SOCS-3 Antibody (OTI3D3) [NBP2-00817]](https://resources.bio-techne.com/images/products/SOCS3-Antibody-3D3-Flow-Cytometry-NBP2-00817-img0001.jpg "Flow Cytometry: SOCS-3 Antibody (OTI3D3) [NBP2-00817]")
Applications for SOCS-3 Antibody (OTI3D3)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SOCS-3
Long Name
Suppressor Of Cytokine Signaling 3
Alternate Names
ATOD4, CIS3, Cish3, SOCS3, SSI-3
Entrez Gene IDs
9021 (Human)
Gene Symbol
SOCS3
Additional SOCS-3 Products
Product Documents for SOCS-3 Antibody (OTI3D3)
Product Specific Notices for SOCS-3 Antibody (OTI3D3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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