SOCS-3 Antibody (SO1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1075
![Western Blot: SOCS-3 Antibody (SO1)BSA Free [NB600-1075]](https://resources.bio-techne.com/images/products/p_13081_1.jpg "Western Blot: SOCS-3 Antibody (SO1)BSA Free [NB600-1075]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Rat
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # SO1
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Full-length SOCS3 protein.
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 24142708).
Specificity
The antibody SO1 reacts with SOCS3, an intracellular cytokine signaling inhibitor.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for SOCS-3 Antibody (SO1) - BSA Free
Western Blot: SOCS-3 Antibody (SO1)BSA Free [NB600-1075]
Western Blot: SOCS3 Antibody (SO1) [NB600-1075] - Fig. 1. Western Blotting analysis (reducing conditions) of whole cell lysate of HeLa human cervix carcinoma cell line. Lane 1: immunostaining with Isotype mouse IgG2b control (PLRV219) Lane 2: immunostaining with anti-SOCS3 (SO1).Applications for SOCS-3 Antibody (SO1) - BSA Free
Application
Recommended Usage
Western Blot
1:1000
Application Notes
Western Blot - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SOCS-3
Long Name
Suppressor Of Cytokine Signaling 3
Alternate Names
ATOD4, CIS3, Cish3, SOCS3, SSI-3
Entrez Gene IDs
89829 (Rat)
Gene Symbol
SOCS3
Additional SOCS-3 Products
Product Documents for SOCS-3 Antibody (SO1) - BSA Free
Product Specific Notices for SOCS-3 Antibody (SO1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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