SOD1/Cu-Zn SOD Antibody (3E5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26309

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3E5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SOD1/Cu-Zn SOD Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human SOD1/Cu-Zn SOD [UniProt P00441]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOD1/Cu-Zn SOD Antibody (3E5)

SOD1/Cu-Zn SOD Antibody (3E5)

Western Blot: SOD1/Cu-Zn SOD Antibody (3E5) [NBP3-26309] -

Western Blot: SOD1/Cu-Zn SOD Antibody (3E5) [NBP3-26309] - Positive Western Blot detected in: 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate.
All lanes: SOD1/Cu-Zn SOD Antibody at 1: 1500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 16 kDa
Observed band size: 18 kDa
SOD1/Cu-Zn SOD Antibody (3E5)

Flow Cytometry: SOD1/Cu-Zn SOD Antibody (3E5) [NBP3-26309] -

Flow Cytometry: SOD1/Cu-Zn SOD Antibody (3E5) [NBP3-26309] - Overlay histogram showing Jurkat cells stained with SOD1/Cu-Zn SOD Antibody (3E5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for SOD1/Cu-Zn SOD Antibody (3E5)

Application
Recommended Usage

Flow Cytometry

1:20-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: SOD1/Cu-Zn SOD

FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS); also called amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Muscle fasciculations are commonly visible. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. ALS is sometimes referred to as Lou Gehrig disease after the famous American baseball player who was diagnosed with the disorder. FALS, the familial form of ALS, accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. The mean age at onset of FALS is 45 years. MISCELLANEOUS: Zinc binding promotes dimerization. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.

Long Name

Superoxide Dismutase-1

Alternate Names

Cu-Zn SOD, CuZn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble

Gene Symbol

SOD1

Additional SOD1/Cu-Zn SOD Products

Product Documents for SOD1/Cu-Zn SOD Antibody (3E5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SOD1/Cu-Zn SOD Antibody (3E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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