SOD1/Cu-Zn SOD Antibody (OTI8B10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47993

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI8B10

Concentration

1 mg/ml

View all SOD1/Cu-Zn SOD Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SOD1 (NP_000445) produced in HEK293T cell.

Specificity

This antibody is specific for Homo sapiens superoxide dismutase 1, soluble (SOD1).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

15.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SOD1/Cu-Zn SOD Antibody (OTI8B10)

Western Blot: SOD1/Cu-Zn SOD Antibody (OTI8B10) [NBP1-47993]

Western Blot: SOD1/Cu-Zn SOD Antibody (OTI8B10) [NBP1-47993]

Western Blot: SOD1/Cu-Zn SOD Antibody (8B10) [NBP1-47993] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Superoxide Dismutase 1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody (OTI8B10) [NBP1-47993]

Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody (OTI8B10) [NBP1-47993]

Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody (8B10) [NBP1-47993] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Superoxide Dismutase 1 mouse monoclonal antibody.
Flow Cytometry: SOD1/Cu-Zn SOD Antibody (OTI8B10) [NBP1-47993]

Flow Cytometry: SOD1/Cu-Zn SOD Antibody (OTI8B10) [NBP1-47993]

Flow Cytometry: SOD1/Cu-Zn SOD Antibody (8B10) [NBP1-47993] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Superoxide Dismutase 1 antibody, and then analyzed by flow cytometry.
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Applications for SOD1/Cu-Zn SOD Antibody (OTI8B10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SOD1/Cu-Zn SOD

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.

Long Name

Superoxide Dismutase-1

Alternate Names

Cu-Zn SOD, CuZn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble

Entrez Gene IDs

6647 (Human)

Gene Symbol

SOD1

Additional SOD1/Cu-Zn SOD Products

Product Documents for SOD1/Cu-Zn SOD Antibody (OTI8B10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SOD1/Cu-Zn SOD Antibody (OTI8B10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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