SOD1/Cu-Zn SOD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31204

Novus Biologicals, part of Bio-Techne
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NBP1-31204
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Cited:

Human, Bovine

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Knockout Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SOD1/Cu-Zn SOD Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SOD1/Cu-Zn SOD. The exact sequence is proprietary.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 30738665). Porcine reactivity reported from a verified customer review.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SOD1/Cu-Zn SOD Antibody

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - Sample (50 ug of whole cell lysate) A: Rat brain 15% SDS PAGE; antibody diluted at 1:1000.
Knockout Validated: SOD1/Cu-Zn SOD Antibody [NBP1-31204]

Knockout Validated: SOD1/Cu-Zn SOD Antibody [NBP1-31204]

Knockout Validated: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody.
View 1 more images

Applications for SOD1/Cu-Zn SOD Antibody

Application
Recommended Usage

Western Blot

1:500-1:10000
Application Notes
IHC-FR-Assay dependent

Reviewed Applications

Read 1 review rated 5 using NBP1-31204 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SOD1/Cu-Zn SOD

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD1), mitochondrial manganese SOD (Mn-SOD/SOD2) and extracellular Cu, Zn-SOD (EC-SOD/SOD3). SOD1 is found in all eukaryotic species as a homodimeric 32-kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80-kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin binding multimer of disulfide linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase(CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.

Long Name

Superoxide Dismutase-1

Alternate Names

Cu-Zn SOD, CuZn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble

Gene Symbol

SOD1

Additional SOD1/Cu-Zn SOD Products

Product Documents for SOD1/Cu-Zn SOD Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SOD1/Cu-Zn SOD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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