SOD1/Cu-Zn SOD Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20534
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat, Alligator, Avian
Predicted:
Chimpanzee (100%). Backed by our 100% Guarantee.
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockout Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human SOD1/Cu-Zn SOD. The exact sequence is proprietary.
Localization
Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SOD1/Cu-Zn SOD Antibody
Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534]
Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NBP2-20534]
Immunocytochemistry/Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - SOD1 antibody detects SOD1 protein at nucleus by immunofluorescent analysis. Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SOD1 protein stained by SOD1 antibody diluted at 1:500. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:200.Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP2-20534]
Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - SOD1 antibody detects SOD1 protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SOD1 antibody dilution: 1:500.Applications for SOD1/Cu-Zn SOD Antibody
Application
Recommended Usage
ELISA
1:1000-1:10000
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
10 - 1:500
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SOD1/Cu-Zn SOD
Long Name
Superoxide Dismutase-1
Alternate Names
Cu-Zn SOD, CuZn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble
Gene Symbol
SOD1
UniProt
Additional SOD1/Cu-Zn SOD Products
Product Documents for SOD1/Cu-Zn SOD Antibody
Product Specific Notices for SOD1/Cu-Zn SOD Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.