SOD2/Mn-SOD [ac Lys68] Antibody (23G5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26531

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26531-100ul
NBP3-26531-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 23G5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SOD2/Mn-SOD Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human SOD2/Mn-SOD [UniProt P04179]

Modification

ac Lys 68

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOD2/Mn-SOD [ac Lys68] Antibody (23G5)

SOD2/Mn-SOD [ac Lys68] Antibody (23G5)

Immunohistochemistry: SOD2/Mn-SOD [ac Lys68] Antibody (23G5) [NBP3-26531] -

Immunohistochemistry: SOD2/Mn-SOD [ac Lys68] Antibody (23G5) [NBP3-26531] - Image of SOD2/Mn-SOD [ac Lys68] Antibody (23G5) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.69% DAB.

Applications for SOD2/Mn-SOD [ac Lys68] Antibody (23G5)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: SOD2/Mn-SOD

Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32 kDa dimer in the cytosol. Mn SOD is an 80 kDa tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from NFmediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDAand nitric oxide-induced neurotoxicity.

Long Name

Superoxide Dismutase-2

Alternate Names

IPO-B, Mn SOD, MnSOD, SOD, Mitochodrial

Gene Symbol

SOD2

Additional SOD2/Mn-SOD Products

Product Documents for SOD2/Mn-SOD [ac Lys68] Antibody (23G5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SOD2/Mn-SOD [ac Lys68] Antibody (23G5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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