SOD2/Mn-SOD Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1969
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Gerbil, Guinea Pig, Hamster, Monkey, Rabbit, Sheep, Xenopus
Cited:
Mouse
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant Human Mn SOD Protein
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 24578415). Immunogen displays sequence identity for non-tested species: Rat, Bovine, Canine, Chicken, Gerbil, Guinea pig, Porcine, Hamster, Monkey, Rabbit, Sheep, Xenopus, Frog and Wood Frog (Rana sylvatica).
Localization
Mitochondria, Mitochondria Matrix
Specificity
Detects approx 25 kDa.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SOD2/Mn-SOD Antibody
Western Blot: SOD2/Mn-SOD Antibody [NB100-1969]
Western Blot: SOD2/Mn-SOD Antibody [NB100-1969] - Western blot analysis of Mouse Brain, Rat Brain, and Rat Brain Membrane showing detection of 24.7kDa SOD (Mn) protein using Rabbit Anti-SOD (Mn) Polyclonal Antibody (NB100-1969). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 ug). Lane 3: Rat Brain (15 ug). Lane 4: Rat Brain Membrane (15 ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody (NB100-1969) at 1:1000 for O/N at 4C. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 24.7kDa.Applications for SOD2/Mn-SOD Antibody
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunohistochemistry
1:100
Immunohistochemistry-Frozen
1:100
Immunohistochemistry-Paraffin
1:100
Immunoprecipitation
1:10 - 1:500
Western Blot
1:5000
Application Notes
0.2 ug/mL of SOD (Mn) Antibody was sufficient for detection of Mn SOD in 20 ug of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-mouse IgG:AP as the secondary Antibody. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24578415).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SOD2/Mn-SOD
Long Name
Superoxide Dismutase-2
Alternate Names
IPO-B, Mn SOD, MnSOD, SOD, Mitochodrial
Gene Symbol
SOD2
UniProt
Additional SOD2/Mn-SOD Products
Product Documents for SOD2/Mn-SOD Antibody
Product Specific Notices for SOD2/Mn-SOD Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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