SOD2/Mn-SOD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20535

Novus Biologicals, part of Bio-Techne
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NBP2-20535
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Alligator, Avian

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Block/Neutralize, IF/IHC, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SOD2/Mn-SOD Antibodies »

Product Specifications

Immunogen

Full length human SOD2/Mn-SOD Recombinant protein.

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SOD2/Mn-SOD Antibody

Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535]

Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535]

Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535]

Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535]

Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP2-20535]

Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP2-20535]

Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP2-20535] - Human breast carcinoma. SOD2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
View 4 more images

Applications for SOD2/Mn-SOD Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000
Application Notes
IHC-FR- Assay dependent

Reviewed Applications

Read 1 review rated 5 using NBP2-20535 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SOD2/Mn-SOD

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Long Name

Superoxide Dismutase-2

Alternate Names

IPO-B, Mn SOD, MnSOD, SOD, Mitochodrial

Gene Symbol

SOD2

Additional SOD2/Mn-SOD Products

Product Documents for SOD2/Mn-SOD Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SOD2/Mn-SOD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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