Sodium Potassium ATPase Alpha 1 Antibody (464.6)
Novus Biologicals, part of Bio-Techne | Catalog # NB300-146
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Canine, Drosophila, Guinea Pig, Primate, Rabbit, Sheep, Xenopus, Yeast
Cited:
Human, Mouse, Rat, Porcine, Bovine, Canine, Frog - Xenopus (African Clawed Frog), Guinea Pig, Insect - Drosophila, Ovine, Rabbit, Yeast
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Block/Neutralize, IF/IHC, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 464.6
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Purified Sodium Potassium ATPase Alpha 1 from rabbit renal outer medulla. [UniProt# Q9N0Z6]
Reactivity Notes
Sheep reactivity reported in scientific literature (PMID: 18424241).
Specificity
This is specific for Na,K-ATPase alpha 1 subunit.
Marker
Plasma Membrane Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Sodium Potassium ATPase Alpha 1 Antibody (464.6)
Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146]
Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Transfected SK-N-AS cells express ICAM-2 transcripts and proteins. ICAM-2 WT and variants localized to cell membranes. Experimental details are included in Methods. Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-261), licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146]
Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - The cellular model of the blood-CSF barrier. Immunofluorescent staining of Na+K+ ATPase and ABCC1, showing the expected respective apical and basolateral membrane localization in the choroidal epithelial cells. The left image is a close up of a single cell to better appreciate the polarity of distribution of the 2 proteins. Nuclei appear in blue. Arrows show the lateral cellular membranes best seen in the z direction by confocal analysis, arrowheads show the basal labeling of ABCC1. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0150945), licensed under a CC-BY license.Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146]
Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Staining of human enodmetrial glands within the uterus using NB300-146. Note the absence of staining in the surrounding myometrial smooth muscle.Applications for Sodium Potassium ATPase Alpha 1 Antibody (464.6)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunocytochemistry/ Immunofluorescence
1:50-1:1000
Immunohistochemistry
1:200
Immunohistochemistry-Frozen
reported in scientific literature (PMID 26941236)
Immunohistochemistry-Paraffin
1:200
Immunoprecipitation
reported in scientific literature (PMID 27748972)
Western Blot
1:1000-1:10000
Application Notes
In Western Blot, a distinct band at ~ 112 kDa is seen. Do not boil the sample prior to loading on the gel for Western Blot (60 degrees Celsius appears to work fine).
Reviewed Applications
Read 4 reviews rated 4.3 using NB300-146 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Sodium Potassium ATPase Alpha 1
Long Name
Sodium/potassium-transporting ATPase subunit alpha-1
Alternate Names
ATP1A1, EC 7.2.2.13
Gene Symbol
ATP1A1
UniProt
Additional Sodium Potassium ATPase Alpha 1 Products
Product Documents for Sodium Potassium ATPase Alpha 1 Antibody (464.6)
Product Specific Notices for Sodium Potassium ATPase Alpha 1 Antibody (464.6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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