Sodium Potassium ATPase Alpha 2 Antibody (JE34-00)

Western Blot: Sodium Potassium ATPase Alpha 2 Antibody (JE34-00) [NBP3-32061]

Western Blot: Sodium Potassium ATPase Alpha 2 Antibody (JE34-00) [NBP3-32061] - Western blot analysis of Sodium Potassium ATPase Alpha 2 on different lysates with Rabbit anti-Sodium Potassium ATPase Alpha 2 antibody (NBP3-32061) at 1/1,000 dilution.

Lane 1: Mouse heart tissue lysate
Lane 2: Rat heart tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 112 kDa
Observed band size: 100 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32061) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Sodium Potassium ATPase Alpha 2 Antibody (JE34-00) [NBP3-32061]

Immunohistochemistry: Sodium Potassium ATPase Alpha 2 Antibody (JE34-00) [NBP3-32061] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Sodium Potassium ATPase Alpha 2 antibody (NBP3-32061) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32061) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: Sodium Potassium ATPase Alpha 2 Antibody (JE34-00) [NBP3-32061]

Flow Cytometry: Sodium Potassium ATPase Alpha 2 Antibody (JE34-00) [NBP3-32061] - Flow cytometric analysis of HeLa cells labeling Sodium Potassium ATPase Alpha 2.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32061, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).