Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A))

Novus Biologicals, part of Bio-Techne | Catalog # NB300-147

Novus Biologicals, part of Bio-Techne
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NB300-147
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Porcine, Bovine, Canine, Chicken, Hamster, Mouse (Negative), Rabbit, Xenopus

Cited:

Human, Mouse, Rat, Porcine, Avian - Chicken, Canine, Frog - Xenopus (African Clawed Frog), Rabbit

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 464.8 (also known as 8A)

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Sodium Potassium ATPase Beta 1 Antibodies »

Product Specifications

Immunogen

Purified Sodium Potassium ATPase Beta 1 from rabbit renal outer medulla. [UniProt# Q9TT37]

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 31356813).

Specificity

This is specific for Na,K-ATPase beta 1 subunit.

Marker

Plasma Membrane Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A))

Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147]

Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147]

Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using NB300-147.
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147]

Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147]

Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Sodium Potassium ATPase Beta 1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147]

Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147]

Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Detection of Na, K-ATPase (beta) in rat kidney homogenates (20 ug)using NB 300-147. Lane 1: 1:2,500 primary. Lane 2: 1:5,000 primary.

Applications for Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A))

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Immunohistochemistry-Paraffin

reported in scientific literature

Western Blot

1:1000-1:5000
Application Notes
This antibody may have a broad banding pattern, typical of glycosylated proteins, at ~35-40 kDa. One may see multiple bands migrating between 35-65 kDa. Do not boil the sample prior to loading on the gel for Western Blot (60 degrees Celsius appears to work fine).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Sodium Potassium ATPase Beta 1

Sodium Potassium ATPase Beta 1 (NKA-beta1), member of X(+)/potassium ATPases subunit beta family, is a single-pass type II membrane protein (transmembrane enzyme) responsible for active reciprocal transport of Na+/K+ ions across plasma membranes, and it contains a larger alpha subunit which catalyze ATP hydrolysis and a smaller beta subunit which is the non-catalytic component of the active enzyme. The alpha subunit (113 kD) contains the binding sites for ATP, Na+ ions, K+ ions, and allosteric sites for inhibitors and activators, and is capable of catalyzing ATP hydrolysis as an essential energy source to transport 3 Na+ ions out of the cell and 2 K+ ions into the cell against membrane ion gradients. The beta subunit (35 kD) is a glycoprotein with isoforms beta1, beta2 and beta3 which are non-catalytic in nature because they lack binding sites for ATP or Na+/K+ ions. In fact, beta subunit is a specific chaperone that assists the biogenesis and corrects membrane insertion of newly synthesized NKA. Moreover, it exerts allosteric activities and by altering the apparent affinity of Na+ /K+ ions as well as by enhancing enzyme turnover rate, NKA-beta1 remotely controls the catalytic function of the enzyme through JY2948-beta1-alpha1 interaction complex. Furthermore, NKA-beta1 may dictate functional NKA concentrations in caveolae, can promote translation efficiency of the alpha subunit and mediate alpha/beta assembly as well as beta-beta homo-oligomerization, and is essential to epithelial polarization, suppression of invasion, and cell motility.

Long Name

Sodium/potassium-transporting ATPase subunit beta-1

Alternate Names

ATP1B, ATP1B1

Entrez Gene IDs

481 (Human); 11931 (Mouse); 25650 (Rat)

Gene Symbol

ATP1B1

UniProt

Q9TT37 (Rabbit)

Additional Sodium Potassium ATPase Beta 1 Products

Product Documents for Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A))

Product Datasheets

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Product Specific Notices for Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A))

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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