Sorbitol Dehydrogenase Antibody (OTI9E7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02126

Novus Biologicals, part of Bio-Techne
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NBP2-02126
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Conjugate
Catalog #
Alexa Fluor 350
NBP2-74288AF350
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Alexa Fluor 405
NBP2-74288AF405
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Alexa Fluor 488
NBP2-74288AF488
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Alexa Fluor 532
NBP2-74288AF532
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Alexa Fluor 594
NBP2-74288AF594
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Alexa Fluor 647
NBP2-74288AF647
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Alexa Fluor 700
NBP2-74288AF700
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Alexa Fluor 750
NBP2-74288AF750
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Allophycocyanin
NBP2-74288APC
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Allophycocyanin/Cy7
NBP2-74288APCCY7
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Biotin
NBP2-74288B
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CoraFluor 1
NBP2-74288CL1
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DyLight 350
NBP2-74288UV
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DyLight 405
NBP2-74288V
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DyLight 488
NBP2-74288G
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DyLight 550
NBP2-74288R
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DyLight 594
NBP2-74288DL594
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DyLight 650
NBP2-74288C
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DyLight 680
NBP2-74288FR
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DyLight 755
NBP2-74288IR
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FITC
NBP2-74288F
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HRP
NBP2-74288H
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Janelia Fluor 525
NBP2-74288JF525
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Janelia Fluor 549
NBP2-74288JF549
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Janelia Fluor 585
NBP2-74288JF585
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Janelia Fluor 635
NBP2-74288JF635
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Janelia Fluor 646
NBP2-74288JF646
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Janelia Fluor 669
NBP2-74288JF669
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mFluor Violet 450 SE
NBP2-74288MFV450
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mFluor Violet 500 SE
NBP2-74288MFV500
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mFluor Violet 610 SE
NBP2-74288MFV610
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PE
NBP2-74288PE
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PE/Atto594
NBP2-74288PEATT594
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PE/Cy5.5
NBP2-74288PECY55
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PE/Cy7
NBP2-74288PECY7
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PerCP
NBP2-74288PCP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI9E7

Concentration

0.9 mg/ml

View all Sorbitol Dehydrogenase Antibodies »

Product Summary for Sorbitol Dehydrogenase Antibody (OTI9E7)

Immunogen

Full length human recombinant protein of human SORD (NP_003095) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

38.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sorbitol Dehydrogenase Antibody (OTI9E7)

Western Blot: Sorbitol Dehydrogenase Antibody (OTI9E7) [NBP2-02126]

Western Blot: Sorbitol Dehydrogenase Antibody (OTI9E7) [NBP2-02126]

Western Blot: Sorbitol Dehydrogenase Antibody (9E7) [NBP2-02126] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Sorbitol Dehydrogenase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Sorbitol Dehydrogenase.
Immunocytochemistry/ Immunofluorescence: Sorbitol Dehydrogenase Antibody (OTI9E7) [NBP2-02126]

Immunocytochemistry/ Immunofluorescence: Sorbitol Dehydrogenase Antibody (OTI9E7) [NBP2-02126]

Immunocytochemistry/Immunofluorescence: Sorbitol Dehydrogenase Antibody (OTI9E7) [NBP2-02126] - Staining of HepG2 cells using anti-SORD mouse monoclonal antibody.
Flow Cytometry: Sorbitol Dehydrogenase Antibody (OTI9E7) [NBP2-02126]

Flow Cytometry: Sorbitol Dehydrogenase Antibody (OTI9E7) [NBP2-02126]

Flow Cytometry: Sorbitol Dehydrogenase Antibody (9E7) [NBP2-02126] - HEK293T cells transfected with either pCMV6-ENTRY Sorbitol Dehydrogenase.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Sorbitol Dehydrogenase mouse monoclonal, and then analyzed by flow cytometry.
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Applications for Sorbitol Dehydrogenase Antibody (OTI9E7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.9 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sorbitol Dehydrogenase

Sorbitol dehydrogenase (SDH), a member of the medium-chain dehydrogenase/reductase protein family and the second enzyme of the polyol pathway of glucose metabolism, converts sorbitol to fructose strictly using NAD(+) as coenzyme. SDH is expressed almost ubiquitously in all mammalian tissues. The enzyme has attracted considerable interest due to its implication in the development of diabetic complications as the polyol pathway is particularly active in hyperglycemic states. Although SORD is closely related to the class I long-chain alcohol dehydrogenases, it differs in substrate specificity, catalyzing polyols such as sorbitol and xylitol but having no activity towards primary alcohols.

Alternate Names

EC 1.1.1, EC 1.1.1.14, L-iditol 2-dehydrogenase, sorbitol dehydrogenase, SORD1

Entrez Gene IDs

6652 (Human)

Gene Symbol

SORD

Additional Sorbitol Dehydrogenase Products

Product Documents for Sorbitol Dehydrogenase Antibody (OTI9E7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sorbitol Dehydrogenase Antibody (OTI9E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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