Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # SOS-01

Format

BSA Free

Concentration

1.0 mg/ml

View all SOS1 Antibodies »

Product Specifications

Immunogen

Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human Sos protein (NM_005633.3)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

The SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDa intracellular protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SOS1 Antibody (SOS-01) - BSA Free

Western Blot: SOS1 Antibody (SOS-01)BSA Free [NB110-68800]

Western Blot: SOS1 Antibody (SOS-01)BSA Free [NB110-68800]

Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt lymphoma cell line
Immunocytochemistry/ Immunofluorescence: SOS1 Antibody (SOS-01) - BSA Free [NB110-68800]

Immunocytochemistry/ Immunofluorescence: SOS1 Antibody (SOS-01) - BSA Free [NB110-68800]

Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence: SOS1 Antibody (SOS-01) - BSA Free [NB110-68800]

Immunocytochemistry/ Immunofluorescence: SOS1 Antibody (SOS-01) - BSA Free [NB110-68800]

Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Applications for SOS1 Antibody (SOS-01) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1 ug/ml
Application Notes
WB: Use under reducing conditions. Antibody purity is > 95% (by SDS-PAGE).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SOS1

The guanine nucleotide exchange factor Sos (Son-of-sevenless) is a complex multidomain protein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionally distinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchange activity of Sos is stimulated by allosteric Ras binding. By another (separable) guanine exchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thus integrates signals that affect both gene expression and cytoskeletal reorganization; the Sos-mediated Ras-activation and Rac activation differ in composition and stability of the formed complex.

Long Name

Son of sevenless homolog 1

Alternate Names

SOS-1

Entrez Gene IDs

6654 (Human); 20662 (Mouse)

Gene Symbol

SOS1

UniProt

Q07889 (Human)

Additional SOS1 Products

Product Documents for SOS1 Antibody (SOS-01) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SOS1 Antibody (SOS-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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