SOX10 Antibody (8E1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26306
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8E1
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human SOX10 [UniProt P56693]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SOX10 Antibody (8E1) - BSA Free
Immunohistochemistry: SOX10 Antibody (8E1) [NBP3-26306] -
Immunohistochemistry: SOX10 Antibody (8E1) [NBP3-26306] - Image of SOX10 Antibody (8E1) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: SOX10 Antibody (8E1) [NBP3-26306] -
Immunohistochemistry: SOX10 Antibody (8E1) [NBP3-26306] - Image of SOX10 Antibody (8E1) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: SOX10 Antibody (8E1) [NBP3-26306] -
Western Blot: SOX10 Antibody (8E1) [NBP3-26306] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate.All lanes: SOX10 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 50, 32 kDa
Observed band size: 60 kDa
Applications for SOX10 Antibody (8E1) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: SOX10
Long Name
Transcription Factor SOX10
Alternate Names
DOM, WS4
Gene Symbol
SOX10
Additional SOX10 Products
Product Documents for SOX10 Antibody (8E1) - BSA Free
Product Specific Notices for SOX10 Antibody (8E1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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