SOX10 Antibody (CL4455) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44116

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-44116
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Catalog #
BSA Free
NBP2-59050
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Feline

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL4455

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SOX10 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P56693, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Feline reactivity reported from a verified customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SOX10 Antibody (CL4455) - Azide and BSA Free

SOX10 Antibody (CL4455) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX10 Antibody [NBP3-44116]

Immunohistochemistry-Paraffin: SOX10 Antibody [NBP3-44116]

Analysis in human cerebral cortex and pancreas tissues using NBP3-44116 antibody. Corresponding SOX10 RNA-seq data are presented for the same tissues.
SOX10 Antibody (CL4455) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX10 Antibody [NBP3-44116]

Immunohistochemistry-Paraffin: SOX10 Antibody [NBP3-44116]

Staining of human breast shows moderate to strong nuclear positivity in glandular cells.
SOX10 Antibody (CL4455) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX10 Antibody [NBP3-44116]

Immunohistochemistry-Paraffin: SOX10 Antibody [NBP3-44116]

Staining of human cerebral cortex shows strong nuclear positivity in glial cells (oligodendrocytes).
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Applications for SOX10 Antibody (CL4455) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Frozen

Validated from a verified customer review.

Immunohistochemistry-Paraffin

1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX10

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

Long Name

Transcription Factor SOX10

Alternate Names

DOM, WS4

Gene Symbol

SOX10

Additional SOX10 Products

Product Documents for SOX10 Antibody (CL4455) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SOX10 Antibody (CL4455) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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