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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SOX10/2311R

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

A recombinant fragment (around aa115-269) of human SOX10 protein (exact sequence is proprietary) (Uniprot: P56693)

Localization

Nuclear

Marker

Melanoma Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79770).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for SOX10 Antibody (SOX10/2311R) - Azide and BSA Free

Western Blot: SOX10 Antibody (SOX10/2311R)Azide and BSA Free [NBP2-79905]

Western Blot: SOX10 Antibody (SOX10/2311R)Azide and BSA Free [NBP2-79905]

Western Blot: SOX10 Antibody (SOX10/2311R) - Azide and BSA Free [NBP2-79905] - Western Blot Analysis of COLO-38 cell lysate using SOX10-Monospecific Recombinant Rabbit Monoclonal Antibody (SOX10/2311R).
Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/2311R) - Azide and BSA Free [NBP2-79905]

Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/2311R) - Azide and BSA Free [NBP2-79905]

Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/2311R) - Azide and BSA Free [NBP2-79905] - Formalin-fixed, paraffin-embedded human Melanoma stained with SOX10-Monospecific Recombinant Rabbit Monoclonal Antibody (SOX10/2311R).
Protein Array: SOX10 Antibody (SOX10/2311R) - Azide and BSA Free [NBP2-79905]

Protein Array: SOX10 Antibody (SOX10/2311R) - Azide and BSA Free [NBP2-79905]

Protein Array: SOX10 Antibody (SOX10/2311R) - Azide and BSA Free [NBP2-79905] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX10 Antibody (SOX10/2311R) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5

Applications for SOX10 Antibody (SOX10/2311R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: SOX10

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

Long Name

Transcription Factor SOX10

Alternate Names

DOM, WS4

Gene Symbol

SOX10

Additional SOX10 Products

Product Documents for SOX10 Antibody (SOX10/2311R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SOX10 Antibody (SOX10/2311R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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