SOX11 Antibody (CL0142)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14782

Novus Biologicals, part of Bio-Techne
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NBP2-14782
NBP2-14782-25ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0142

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SOX11 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FMVWSKIERRKIMEQSPDMHNAEISKRLGKRWKMLKDSEKIPFIREAERLRLKHMADYPDYKYRPRKKPKMDPSAKPSASQSPEKSAAGGGGGSAGGGAGGAKTSKGSSKK

Epitope

IPFIREAERL

Reactivity Notes

Expected to cross react based on sequence identity: Rat (82%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SOX11 Antibody (CL0142)

Western Blot: SOX11 Antibody (CL0142) [NBP2-14782]

Western Blot: SOX11 Antibody (CL0142) [NBP2-14782]

Western Blot: SOX11 Antibody (CL0142) [NBP2-14782] - Analysis in control (vector only transfected HEK293T lysate) and sOX11 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: SOX11 Antibody (CL0142) [NBP2-14782]

Immunocytochemistry/ Immunofluorescence: SOX11 Antibody (CL0142) [NBP2-14782]

Immunocytochemistry/Immunofluorescence: SOX11 Antibody (CL0142) [NBP2-14782] - Staining of RH-30 cells using the Anti-SOX11 monoclonal antibody, showing specific staining in the nucleus and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782]

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782]

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782] - Staining of eye in mouse embryo E14 retina shows moderate nuclear positivity in developing retina.
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Applications for SOX11 Antibody (CL0142)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Simple Western

5 ug/ml

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX11

SOX11 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The protein may function in the developing nervous system and play a role in tumorigenesis.

Long Name

Transcription Factor SRY (Sex Determining Region Y)-box 11

Alternate Names

SRY (sex determining region Y)-box 11, SRY (sex-determining region Y)-box 11, SRY-related HMG-box gene 11, transcription factor SOX-11

Gene Symbol

SOX11

Additional SOX11 Products

Product Documents for SOX11 Antibody (CL0142)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SOX11 Antibody (CL0142)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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